22nd Enzyme Mechanisms Conference
Sunday January 2 - Thursday January 6, 2011
List of Posters
Authors of ODD numbered posters should be available to present and discuss their poster on Monday January 3rd.
Authors of EVEN numbered posters should be available to present and discuss their poster on Tuesday January 4th.
P1. From mechanistic Enzymology to Biology: Deciphering the Function of RubisCO-like Proteins
Tobias J. Erb and John A. Gerlt
P2. Lid dynamics in catalysis: A free energy rearrangement
Troy Johnson and Todd Holyoak
P3. Establishing Structural Diversity in Ergot Alkaloids by an Old Yellow Enzyme Homologue
Johnathan Z. Cheng, Christine M. Coyle, Daniel G. Panaccione and Sarah E. O’Connor
P4. Substituent Effects on the Reaction of β-Benzoylalanines with Pseudomonas fluorescens Kynureninase
Sunil Kumar, Vijay B. Gawandi, Nicholas Capito and Robert S. Phillips
P5. Analysis of the peroxiredoxin family: Using active site structure and sequence information for global classification and residue analysis
Kimberly J. Nelson, Laura Soito, Stacy T. Knutson, Leslie B. Poole and Jacquelyn S. Fetrow
P6. A Chemico-Biological Rationale for the Use of Selenocysteine in a Redox Active Enzyme: Focus on Mammalian Thioredoxin Reductase
Gregg W. Snider and Robert J. Hondal
P7. The Mechano-Enzymatic Function of Selenocysteine In Thioredoxin Reductase
Adam P. Lothrop and Robert J. Hondal
P8. Contribution of 5’ leader of the Precursor tRNA toward Recognition and Catalysis by Bacterial RNase P
Wan Hsin Lim and Carol A. Fierke
P9. Glycosyltransferase Dynamics: Studying the Model System of MshA from Corynebacterium glutamicum
Jacquelyn S. Turri and Patrick A. Frantom
P10. Glutamine-Dependent Asparagine Synthetase from Plasmodium berghei
Yong-Mo Ahn and Nigel G. J. Richards
P11. Biochemical and Biophysical Characterization of Human Pancreatic Glucokinase
Mioara Larion and Brian G. Miller
P12. pH Dependency of the Pericyclic Reactions Catalyzed by the Isochorismate-Pyruvate Lyase from Pseudomonas aeruginosa
Jose Olucha, Qianyi Luo and Audrey L. Lamb
P13. Directed Evolution of a Thermostable Quorum-Quenching Lactonase from the Amidohydrolase Superfamily
Jeng Yeong Chow, Bo Xue, Robert C. Robinson and Wen Shan Yew
P14. Studying the Substrate Tolerance of 4-Coumarate-CoA ligase from Streptomyces Coelicolor
Maybelle K. Go, Lim Yan Ping and Yew Wen Shan
P15. Synthesis and Screening a Haloacetamide Containing Library
Justin E. Jones, Jessica Slack, Corey P. Causey and Paul R. Thompson
P16. Activity-Based Protein Profiling of Protein Arginine Methyltransferase 1
Obiamaka Obianyo, Corey P. Causey and Paul R. Thompson
P17. Characterizing the Mechanism of PRMT1 Catalysis Using Site Directed Mutagenesis
Heather Rust and Paul Thompson
P18. The Development and Use of Clickable Activity Based Protein Profiling Agents for Protein Arginine Deiminase 4
Jessica L. Slack, Corey P. Causey, Yuan Luo and Paul R. Thompson
P19. Control of Mitochondrial Biogenesis by Mitochondrial Dynamics
Tianzheng Yu and Yisang Yoon
P20. Kinetic Analysis of Malate Synthase from Mycobacterium tuberculosis
Christine Quartararo and John S. Blanchard
P21. Hydroxymethylglutaryl-CoA lyase (HMGCL): Functional insights from structures of HMGCL-Mg++-hydroxyglutaryl-CoA and HMGCL R41M-Mg++-HMG-CoA ternary complexes
Henry M. Miziorko, Zhuji Fu, Jennifer A. Runquist and Jung-Ja P. Kim
P22. A Heme-dependent Tyrosine Hydroxylase
Katherine L. Connor and Barbara Gerratana
P23. The Structure of a C-3'-Methyltransferase Required for the Biosynthesis of dTetronitrose
Nathan A. Bruender, James B. Thoden, Manpreet Kaur, Marie K. Avey and Hazel M. Holden
P24. Structural and Functional Studies of the Dehydrogenases Required for the Biosynthesis of 2,3-Diacetamido-2,3-dideoxy-d-mannuronic Acid
James B. Thoden and Hazel M. Holden
P25. Design of Non-covalent Small Molecule Inhibitors for Granzyme B
Mi-Sun Kim, Dean A Heathcote, Philip G Ashton-Rickardt and James P. Snyder
P26. Long-range dynamic networks in the function and fidelity of poliovirus RNA-dependent RNA polymerase
David D. Boehr, Xiaorong Yang and Jesse L. Welch
P27. Kinetically Controlled Drug Resistance: How Penicillium brevicompactum Survives
Xin Sun, Bjarne Gram Hansen and Lizbeth Hedstrom
P28. Spectroscopic Studies on the [4Fe-4S] Cluster in Adenosine 5-phosphosulfate Reductase from Mycobacterium tuberculosis
Devayani P. Bhave, Jiyoung A. Hong, Michael Lee, Wei Jiang, Carsten Krebs and Kate S. Carroll
P29. Dihydrofolate reductase: A correlation between the donor-acceptor distance and its fluctuation to the catalyzed hydride transfer
Vanja Stojkovic, Laura Perissinoti, Stephen J. Benkovic and Amnon Kohen
P30. Characterization of the Carboxybiotin Intermediate in Reactions of Pyruvate Carboxylase.
Tonya N. Zeczycki, Martin St. Maurice, Sarawut Jitrapakdee, John C. Wallace, Paul V. Attwood and W. W. Cleland
P31. Mechanistic Studies of Methylphosphonate Synthase, a Non-Heme Fe- Dependent Dioxygenase Linked to the Marine Carbon-Phosphorus Cycle
Heather A. Cooke and Wilfred A. van der Donk
P32. Structural and Functional Significance of the Unstructured Loop Region of Alkanesulfonate Monooxygenase
Jingyuan Xiong and Holly R Ellis
P33. Kinetic and Chemical Mechanisms of Homocitrate Synthase from Thermus thermophilus
Vidya Prasanna Kumar and Paul F. Cook
P34. Solvent Isotope Effects on the Desulfonation Mechanism of the Two-Component Alkanesulfonate Monooxygenase System
John M. Robbins and Holly R. Ellis
P35. Developing polyketide-based inhibitors targeting 3-dehydroquinate dehydratase in the shikimate biosynthesis pathway.
Vivian W. N. Cheung, Maybelle K. Go, Robert C. Robinson and Wen Shan Yew
P36. Structural studies of MppR: An enzyme of unknown function from enduracididine biosynthesis
Tyler Voegtline, Bilal H. Sayyed, Eric D. Lund and Nicholas R. Silvaggi
P37. On the Analysis of Ligand Displacement Kinetics when a Probe is Rate Contributing
Hidehisa Iwata and Mark S. Hixon
P38. Discovery and design of inhibitors of dimethylarginine dimethylaminohydrolase inhibitors by high throughput screening
Thomas Linsky, Corey Johnson, Yun Wang and Walter Fast
P39. Substrate Specificity Amongst β-Ketoacyl-ACP Synthases
Janine Borgaro, Andrew Chang and Peter J. Tonge
P40. Coenzyme-induced conformational changes in a flavin-dependent N-hydroxylating monooxygenase
Pablo Sobrado, Wyatt Chocklett, Elvira Romero and Michelle Oppenheimer
P41. Histidine 252 in Adenosine 5'-phosphosulfate Reductase Is Required for Substrate Binding
Jiyoung A. Hong and Kate S. Carroll
P42. Characterization and purification of 3-deoxy-d-manno-oct-2-ulosonic acid hydroxylase, KdoO from Burkholderia ambifaria
Hak Suk Chung and Christian R. H. Raetz
P43. 4-Oxalocrotonate Decarboxylase and Vinylpyruvate Hydratase from Leptothrix cholodnii SP-6 May Act on a Chlorinated Form of the Substrate: Analysis and Quantitation
Stephanie Taylor and Christian P. Whitman
P44. Identification of the allosteric response element(s) in the active site of mammalian pyruvate kinase isozymes
James Urness, Cody Timmons, Kelly Thuet and Aron W. Fenton
P45. Characterization of substrate binding loop of 3α-hydroxysteroid dehydrogenase/carbonyl reductase
Chi-Ching Hwang, Yu-Mei Su and Hsin-Wei Chen
P46. Promotion of Oligomeric DNA Polymerase Complex Formation Dictates Alternative Enzymatic Activities in Archaea
Michael A. Trakselis, Zhongfeng Zuo, Hsiang-Kai Lin and Robert Bauer
P47. MshB – A Cambialistic Metallohydrolase?
M. Hernick, X. Huang and E. Kocabas
P48. Probing the mechanism of DpgC, a unique cofactor and metal-free dioxygenase involved in the biosynthesis of vancomycin
Heather Condurso, Elisha Fielding, Paul Widboom and Steve Bruner
P49. Functional Promiscuity of Rhodospirillum rubrum RuBisCO for RuBisCO-Like Protein Reactions
Benjamin Warlick and John A. Gerlt
P50. Thymidylate Synthase Catalyzed H-Transfers: Role of Tunneling and Dynamics in Different Enzyme catalyzed C-H activations
Zhen Wang and Amnon Kohen
P51. Elucidating the Energetics of Active Site Loop Motion in Formyl-CoA:Oxalate CoA Transferase
Sangbae Lee, Mengen Chen, Wei Yang and Nigel G. J. Richards
P52. Discovery of a New Class of Quiescent Affinity Label: 4-Halopyridines Inactivate Dimethylarginine Dimethylaminohydrolase
Corey M. Johnson, Thomas W. Linsky, Joyce Er and Walter Fast
P53. Mechanism of a disulfide-generating enzyme: the Quiescin-sulfhydryl oxidase from Trypanosoma brucei
Vamsi K. Kodali, Benjamin A. Israel and Colin Thorpe
P54. Two mechanisms for UDP-2,3-diacylglucosamine hydrolysis in lipid A biosynthesis
Hayley E. Young, Louis E. Metzger IV, John K. Lee, Robert M. Stroud and Christian R. H. Raetz
P55. Enzymatic Study and Chemical Inhibitor of the Histone Acetyltransferase Tip60
Jiang Wu, Nan Xie, Emilia N. Elangwe, Chao Yang and Yujun George Zheng
P56. Engineering E. coli for new chemical function
Brooks B. Bond-Watts and Michelle C. Y. Chang
P57. Characterization of enzymes involved in the degradation of 2-aminoethylphosphonate in Sinorhizobium meliloti: phosphonoacetaldehyde dehydrogenase PhnY and phosphonoacetate hydrolase PhnA
Svetlana A. Borisova, Vinayak Agarwal, Nurul Zulkepli, Satish K. Nair, Wilfred A. van der Donk and William W. Metcalf
P58. Suppression of a polymerase general acid mutant: Consequences on replication speed and polymerase fidelity
Jamie J. Arnold, Madhumita Yennawar, Yan Zhao, Eric D. Smidansky and Craig E. Cameron
P59. Identification of PODANSg2158 from P. anserina as a Nitroalkane Oxidase and Comparison with the Enzyme from F. oxysporum
José R. Tormos and Paul F. Fitzpatrick
P60. Inhibition by Nitric Oxide of Catalysis by Oxalate Decarboxylase
Mario Edgar G. Moral, Chingkuang K. Tu, Witcha Imaram, Alexander Angerhofer, David N. Silverman and Nigel G. J. Richards
P61. Studies on the kinetic sequence of the Mycobacterium tuberculosis DHFR
Clarissa M. Czekster, An Vandemeulebroucke and John S. Blanchard
P62. Design, Synthesis and Evaluation of Quinolone-based Inhibitors of E. coli PBP5
A. G. Shilabin, B. Jayaram and R. F. Pratt
P63. Inhibition of Bacterial Phosphopantothenoylcysteine Synthetase
James D. Patrone, Jiangwei Yao, Jennifer L. Meagher, Jeanne A. Stuckey, and Garry D. Dotson
P64. Determination of the crystal structure of a two-domain NRPS fusion protein, EntEB, from the enterobactin synthetic cluster of Escherichia coli
Jesse A. Sundlov, Eric J. Drake, Courtney C. Aldrich and Andrew M. Gulick
P65. Probing the Mechanism of Phenylalanine Hydroxylase Using Rapid-Mixing Techniques with High-Resolution Mass Spectrometry
Kenneth M. Roberts and Paul F. Fitzpatrick
P66. Identification of the Enzyme That Catalyzes the Final Step in the Biosynthesis of Queuosine
Zachary D. Miles, Reid M. McCarty, Gabriella Molnar and Vahe Bandarian
P67. The chemistry of DXP synthase: Implications for selective inhibitor design
Francine Morris and Caren L. Freel Meyers
P68. The Parts Aren’t Interchangeable: Installing a Proton-Transfer Network into a Class 1A Dihydroorotate Dehydrogenase
Claudia A. McDonald and Bruce A. Palfey
P69. Characterization of the Putative Diels-Alderase LovB CON
Joel J. Bruegger, Brian Ames, Suzanne Ma, Jesse Li, John Vederas, Yi Tang and Sheryl Tsai
P70. Structural Analysis of the Isochorismate-Pyruvate Lyase from Pseudomonas aeruginosa
Andrew Ouellette, Jose Olucha, Qianyi Luo, Roberto De Guzman and Audrey L. Lamb
P71. A Site Under Selective Pressure in the LexA Repressor is Required for Control of the DNA Damage Response
D. C. Marciano, P. Katsonis, A. K. Adikesavan, C. Herman and O. Lichtarge
P72. Characterization of Ceriporiopsis subvermispora Bicupin Oxalate Oxidase Expressed In Pichia pastoris
Kelsey Uberto, Eric Hoffer , Patricia Moussatche, Alexander Angerhofer, Witcha Imaram, Nigel G. J. Richards and Ellen W. Moomaw
P73. Chemical Model for the Action of a Single Catalytic Residue, Arginine, at Active Site of Uroporphyrinogen Decarboxylase
Charles A. Lewis, Jr. and Richard Wolfenden
P74. Auxiliary Proteins in Alginate Maturation
Emma K. Farrell, Krista Arnett, Howard Robinson, Joel T. Weadge, P. Lynne Howell and Peter A. Tipton
P75. Assessment of Invertebrate Thiaminases by Mass Spectrometry
Ivy Bergquist, Randy L. Sears Jr., Lisa C. Harding, Vatsal M. Patel, Seetha R. Thummuru and John V. Schloss
P76. Mechanism-based Affinity Capture of Sirtuins
Jessica Falco, Yana Cen, Dou Yeon Youn, Ping Xu and Anthony A. Sauve
P77. Confirmation of the mechanism of Unsaturated Glucuronyl Hydrolase
S. A. K. Jongkees and S. G. Withers
P78. Design, synthesis and evaluation of new -lactamase inhibitors
Ronak Tilvawala and R. F. Pratt
P79. Boronate Inhibitors of Penicillin Binding Proteins: Evaluation in vitro, in Cell Membranes, and in vivo
Liudmila Dzhekieva and R. F. Pratt
P80. Human mitochondrial RNA polymerase: Evaluation of the single-nucleotide-addition cycle on synthetic RNA/DNA scaffolds
Eric D. Smidansky, Jamie J. Arnold, Ibrahim M. Moustafa, Shelley L. Reynolds and Craig E. Cameron
P81. Dynamics of Conformational Heterogeneity within the Michaelis Complex of Lactate Dehydrogenase
Ruel Z. Desamero, Beining Nie, Nick Zhadin, Hua Deng and Robert Callender
P82. An Unprecedented Mechanism in Thiamine-Dependent Enzyme Catalysis: DXP Synthase
Leighanne A. Brammer, Jessica Mott, Herschel Wade and Caren Freel Meyers
P83. Ultra High Resolution Analysis of CTX-M β-Lactamase to study catalysis and discover novel inhibitors
Derek Nichols and Yu Chen
P84. Characterization and Lead Development of inhibitors of Dimethylarginine Dimethylaminohydrolase
Gayle Burstein , Thomas W. Linsky and Walter Fast
P85. Transition State of Human Nicotinamide Phosphoribosyltransferase
Emmanuel S. Burgos, Mathew J. Vetticatt and Vern L. Schramm
P86. 15N Isotope Effects on NH3 for the reaction of NAD+ Synthetase (M. tuberculosis)
Laurie A. Reinhardt, Kaiti Chang, Barbara Gerratana and W. Wallace Cleland
P87. Computational design of enzymes for novel carbon-carbon bond formation reactions
Asha Chaubey, Elizabeth Noey and K. N. Houk
P88. Exploring The Inhibitory Effect Of The Hydrazine Functionality On The Histone Demethylase LSD1
Shonoi A. Barnett, Lindsay Avery, Rong Huang and Philip A. Cole
P89. Inhibition of Serine/Amine Amidohydrolases by O-Aryloxycarbonyl Hydroxamates and Oxathiazol-2-ones: Penicillin acylase and -lactamases
S. A. Adediran and R. F. Pratt
P90. Substrate specificity of penicillin-binding proteins
V. V. Nemmara and R. F. Pratt
P91. Coupled pKa Shift Between Conserved Cysteine and Aspartate Plays Key Role in Protein Splicing
Zhenming Du, Yangzhong Liu and Chunyu Wang
P92. Enzymatic Mechanism of Protein Arginine Methyltransferase (PRMT) Probed by Stopped-Flow Fluorescence
You Feng and Y. George Zheng
P93. Global Dynamics in Catalysis by Horse Liver Alcohol Dehydrogenase?
Bryce V. Plapp, Atsushi Yahashiri, Rachel S. Wallace, Karthik K. Shanmuganatham and Timothy J. Herdendorf
P94. DNA translocation dynamics revealed by single molecule nucleotide binding kinetics
Matthew Kellinger, Jonas Korlach and Kenneth A. Johnson
P95. Allosteric Regulation of α-Isopropylmalate Synthase from Mycobacterium tuberculosis
Ashley K. Casey, Jordyn L. Johnson and Patrick A. Frantom
P96. Enzymology as a Tool for Crystallography within the CSGID
Misty L. Kuhn, Magdalena M. Makowska-Grzyska, George Minasov, Ludmilla Shuvalova, Ievgeniia Dubrovska, James Winsor, Andrzej Joachimiak and Wayne Anderson
P97. High-Throughput Screening for Selective Inhibitors of N5-Carboxyaminoimidazole Ribonucleotide Mutase
Hanumantharao Paritala, Maria Fawaz, James B. Thoden, Hazel M. Holden and Steven M. Firestine
P98. Long-distance relationships: Communication between the active site lid and a remote loop in PEPCK
Sarah M. Holyoak and Todd Holyoak
P99. Site-specific installation of two lysine derivatives in histone H3
Wenshe Liu, Ying Huang and Wei Wan
P100. Targeting thymidylate biosynthesis for the development of new antibiotics
Eric M. Koehn, Scott A. Lesley, Irimpan I. Mathews and Amnon Kohen
P101. 15N, 13C Primary and 18O Nucleophilic Kinetic Isotope Effects for the Reaction Catalyzed by Sir2
Mark A. Anderson, W. W. Cleland, Brian C. Smith, Dawei Chen and John M. Denu
P102. Functional Assignment of the N245 Residue of 4-Hydroxyphenylpyruvate Dioxygenase from Streptomyces avermitilis: A Key Residue in Hydroxylation Regiospecificity
June Brownlee, Dhara Shah, Judith Bates and Graham R. Moran
P103. Small-molecule inhibitor of a human deubiquitylating enzyme complex
Junjun Chen, Thomas Dexheimer, Yongxing Ai, Anton Simeonov and Zhihao Zhuang
P104. Insights into the chemical mechanism of eukaryotic UDP-galactopyranose mutases
Michelle Oppenheimer, Jin Qi, Janice Llanos-Valezquez and Pablo Sobrado
P105. Substrate Identification for the HDAC orthologue Rpd3 using in vivo photo-crosslinking
Noah Wolfson, Jody Lancia, Anna Mapp and Carol Fierke
P106. Kinetically Controlled Allosteric Activation: Potassium creates a ball and socket joint in IMP Dehdyrogenase
Thomas V. Riera, Lianqing Zheng, Helen R. Josephine, Donghong Min, Wei Yang and Lizbeth Hedstrom
P107. The Human Hotdog-fold Protein THEM4 is Both a Protein Kinase Bα (Akt1) Regulator and a Fatty Acyl-CoA Thioesterase
Hong Zhao, Anthony Choudhry, Jeffery D. Carson, Michael Schaber, Peter J. Tummino, Lusong Luo, Marco Bossoffi and Debra Dunaway-Mariano
P108. Insights Into the Divergence of Function in the Hotdog-fold Protein Products of Gene Duplicates II: A Case Study of the E. coli Hotdog Thioesterases YbdB (EntH) and YdiI
John Latham, Rui Wu, Danqi Chen, Karen Allen and Debra Dunaway-Mariano
P109. Biotin synthase: How iron-sulfur clusters are used to make carbon-sulfur bonds
Andrew M. Taylor, Christine E. Farrar, Michael R. Reyda, Corey J. Fugate and Joseph T. Jarrett
P110. Thermodynamic Profiles for Tight Binding Inhibitors to MTANs
Keisha Thomas and Vern L. Schramm
P111. Replica Path Determination of Bortezomib Deboronation
Joseph D. Larkin, Peng Tao, H. Lee Woodcock, Milan Hodoscek and Bernard R. Brooks
P112. Biosynthesis of 1-Deoxynojirimycin
Lorraine Clark and Nicole Horenstein
P113. Detailed Kinetic Analysis of Irreversible EGFR Inhibitors as a Method of Driving Drug Design
Phillip A. Schwartz and Brion W. Murray
P114. Investigation of Substrate Recognition in the Yeast Candida albicans Protein Prenyltransferases
Elaina A. Zverina and Carol A. Fierke
P115. Role of dynamics in tuning fidelity of RNA-dependent RNA polymerase elucidated by molecular dynamics simulation
Ibrahim M. Moustafa, Coray M. Colina and Craig E. Cameron
P116. The Human Estrogen Sulfotransferase - a Half-Site Reactive Enzyme
Meihao Sun and Thomas S. Leyh
P117. Real-time Fluorescence Polarization Assay for Steady-state Kinetics and Screening for Inhibitors of Bacterial RNase P
Xin Liu and Carol Fierke
P118. A Critical Pleiotropic Interaction Governing the Specificity of 5-epi-aristolocholene Synthase and its Evolutionary Implications
Troy Wymore and Charles L. Brooks III
P119. Pre-steady state analysis of trans-3-chloroacrylic acid dehalogenase
Jamison P. Huddleston, Gottfried K. Schroeder, Kenneth A. Johnson and Christian P. Whitman
P120. The rate-limiting step for the second-order hydrolysis of many substrates by acetylcholinesterase (kcat/KM) is gated entry to the catalytic triad
Jeffrey T. Auletta, Veena Beri, Robert Chapman, Juanita F. Wood, Abdul H. Fauq and Terrone L. Rosenberry
P121. Enzyme Diversity and Evolution among the Enolase Superfamily: Understanding Sequence/Function/Structure Relationship
Ayano Sakai, Alexander A. Fedorov, Elena V. Fedorov, Steven C. Almo and John A. Gerlt
P122. QueE, a New Member of the Radical SAM Superfamily, is Involved in the Biosynthesis of 7-deazapurines
Reid M. McCarty and Vahe Bandarian
P123. Evidence for a phosphorylated enzyme intermediate in the reaction catalyzed by methylthioribose-1-phosphate isomerase
Vamsee Veeramachineni and Andrew S. Murkin
P124. Reactions of Propiolic Acid with Tautomerase Superfamily Dehalogenases: Analysis and Implications
Gottfried K. Schroeder, William H. Johnson, Jr., Jamison P. Huddleston, Kenneth A. Johnson, and Christian P. Whitman
P125. Structural evidence that peroxiredoxin catalytic power is based on transition state stabilization
Leslie B. Poole, Andrea Hall, Derek Parsonage and P. Andrew Karplus
P126. Selective Inhibition of Ras Function in Live Cells using Caged Farnesyltransferase Inhibitors
Daniel Abate-Pella, Nicholette A. Zeliadt, Timothy M. Dore, David A. Blank, Elizabeth V. Wattenberg and Mark D. Distefano
P127. Investigation of the HAD Superfamily Phosphatidic Acid Phosphatase Human Lipin1 and its HAD Superfamily Phosphatase Regulator Dullard
Jeremiah Farelli, Rui Wu, Megan Garland, Li Zheng, Debra Dunaway-Mariano and Karen Allen
P128. Insights Into the Divergence of Function in the Hotdog-fold Protein Products of Gene Duplicates II: A Case Study of the Human Hotdog Thioesterases hTHEM5 and hTHEM4
Hong Zhao, Dennis Dominguez, Kap Lam, Osnat Herzberg and Debra Dunaway-Mariano
P129. Identification and Structure Determination of Novel Adenine Deaminase Enzymes
Alissa M. Goble, Zhening Zhang, Subramanyam Swaminathan and Frank M. Raushel
P130. A Study on the Transesterification of Methyl Aryl Phosphorothioates in Methanol Promoted by Cd(II), Mn(II) and a Synthetic Pd(II) Complex
David R. Edwards, Alexei Neverov and R. Stan Brown
P131. Impact of temperature on the time required for primordial chemistry and enzyme evolution
Randy B. Stockbridge, Charles A. Lewis, Jr., Yuan Yang and Richard Wolfenden
P132. Is Leu403 an Absolute in Maintaining the “V” Conformation of Thiamin Diphosphate in Benzoylformate Decarboxylase?
Forest H. Andrews and Michael J. McLeish
P133. Structure and Mechanism of Benzaldehyde Dehydrogenase: A Class 3 Aldehyde Dehydrogenase from Pseudomonas putida
Michael J. McLeish, Shreenath Prasad, Megan P.D. Zahniser, Gregory Petsko and Dagmar Ringe
P134. Enzymatic Extender Unit Generation for In Vitro Polyketide Synthase Reactions: Structural and Functional Showcasing of Streptomyces coelicolor MatB
Amanda Jane Hughes and Adrian Keatinge-Clay
P135. Diketide Substrates Probe the Biocatalytic Potential of Modular Polyketide Synthase Ketoreductases
Shawn K. Piasecki, Clint A. Taylor, June Liu, Jianting Zheng, Joshua F. Detelich, Arkady Komsoukaniants, Dionicio R. Siegel and Adrian T. Keatinge-Clay
P136. Guilt by association: Functional annotation of TM0486 from Thermotoga maritime by identification of its bound ligands
Mark J. Snider, Brad A. Palanski and Zachary Rotter
P137. Aerobic catabolism of nicotinic acid in Bordetella bronchiseptica: Mechanistic studies of NicC, NicX and NicF
Mark J. Snider, Eric Sullivan, Matthew Henke, Virginia Kincaid, Ryan Story and Roger Rowlett
P138. Challenging a Paradigm. Catalysis and Inhibition in Cysteine Proteases
Michael Shokhen, Netaly Khazanov and Amnon Albeck
P139. Necrotic cell death: Is it manageable?
Keren Schultz, Boris Khalfin, Ilana Nathan and Amnon Albeck
P140. Computational Investigation of the Cisoid Cyclization Pathway of 5-epi-aristolocholene synthase
Carlos A. Ramirez-Mondragon and Troy Wymore
P141. Computational Study of Glutamate-γ-Semialdehyde Oxidation Catalyzed by Thermus Thermophilus Gamma Glutamyl Semialdehyde Dehydrogenase
Nikolay Simakov and Troy Wymore
P142. Structural investigation of a novel NRPS cluster from Pseudomonas aeruginosa
Carter A. Mitchell and Andrew M. Gulick
P143. Computational Exploration of Reaction Mechanism: Energetics Of DD-Peptidase Using A Hybrid QM/MM With The Off-Path Simulation
Justin K. White, Jacquline Hargis and H. Lee Woodcock
P144. The role of Phe161 in the mechanism of NADH-dependent persulfide reductase from Shewanella loihica PV-4
Kyu Hyun Lee and E. J. Crane
P145. On the accuracy of NMR-measured protein pKa values
Helen Webb, Barbara Mary Tynan-Connolly, Gregory M. Lee, Damien Farrell, Fergal O'Meara, Chresten R. Søndergaard Kaare Teilum, Chandralal Hewage, Lawrence P. McIntosh and Jens Erik Nielsen
P146. Crystal Structures of Both Unbound and ATP Analog-Bound Forms of E. coli Anhydromuramic Acid Kinase, An Important Enzyme in Peptidoglycan Recycling
C. Leigh Allen, Bradley M. Hover, Nathan I. Nicely and Dewey G. McCafferty
P147. Identifying the Protein Targets of Epidermal Growth Factor-Induced Hydrogen Peroxide
Candice E. Paulsen and Kate S. Carroll
P148. Substrate Recognition and Catalytic Mechanism of MenB, the 1,4-Dihydroxynaphthoate Synthase in the Menaquinone Biosynthesis Pathway
Huei-Jiun Li, Xiaokai Li, Nina Liu, Huaning Zhang and Peter J. Tonge
P149. Insights into the Mechanism of a Bacterial Quercetin 2,3 Dioxygenase
Sara Bowen, Matthew R. Schaab and Wilson A. Francisco
P150. Enzymatic Incorporation of Bioorthogonal Function into Proteins: New Strategies for Microarray Construction
Rajesh Viswanathan and Alden E. Voelker
P151. Kinetic Studies of the Bi-Functional Enzyme a-Aminoadipate-Semialdehyde-Synthase from Bovine Liver
William E. Karsten and Paul F. Cook
Organizing Committee
John Richard, Chair SUNY Buffalo
Kay Ahn Pfizer Global Research and Development
Tadhg Begley Texas A&M University
Debra Dunaway-Mariano University of New Mexico
Francine Perler New England Biolabs
Richard Wolfenden University of North Carolina at Chapel Hill
John P. Richard
Tina L. Amyes
University at Buffalo
Department of Chemistry
Buffalo, NY 14260, USA
(716) 645 4232 (John)
(716) 645 4231 (Tina)
Fax: (716) 645 6963