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22nd Enzyme Mechanisms Conference

Sunday January 2 - Thursday January 6, 2011

List of Posters


Authors of ODD numbered posters should be available to present and discuss their poster on Monday January 3rd.

Authors of EVEN numbered posters should be available to present and discuss their poster on Tuesday January 4th
.

P1. From mechanistic Enzymology to Biology: Deciphering the Function of RubisCO-like Proteins
Tobias J. Erb and John A. Gerlt

P2. Lid dynamics in catalysis: A free energy rearrangement
Troy Johnson and Todd Holyoak

P3. Establishing Structural Diversity in Ergot Alkaloids by an Old Yellow Enzyme Homologue

Johnathan Z. Cheng, Christine M. Coyle, Daniel G. Panaccione and Sarah E. O’Connor

P4. Substituent Effects on the Reaction of
β-Benzoylalanines with Pseudomonas fluorescens Kynureninase
Sunil Kumar, Vijay B. Gawandi, Nicholas Capito and
Robert S. Phillips

P5. Analysis of the peroxiredoxin family: Using active site structure and sequence information for global classification and residue analysis

Kimberly J. Nelson, Laura Soito, Stacy T. Knutson, Leslie B. Poole and Jacquelyn S. Fetrow

P6. A Chemico-Biological Rationale for the Use of Selenocysteine in a Redox Active Enzyme: Focus on Mammalian Thioredoxin Reductase

Gregg W. Snider and Robert J. Hondal

P7. The Mechano-Enzymatic Function of Selenocysteine In Thioredoxin Reductase

Adam P. Lothrop and Robert J. Hondal

P8. Contribution of 5’ leader of the Precursor tRNA toward Recognition and Catalysis by Bacterial RNase P

Wan Hsin Lim and Carol A. Fierke

P9. Glycosyltransferase Dynamics: Studying the Model System of MshA from
Corynebacterium glutamicum
Jacquelyn S. Turri and Patrick A. Frantom

P10. Glutamine-Dependent Asparagine Synthetase from
Plasmodium berghei
Yong-Mo Ahn and Nigel G. J. Richards

P11. Biochemical and Biophysical Characterization of Human Pancreatic Glucokinase

Mioara Larion and Brian G. Miller

P12. pH Dependency of the Pericyclic Reactions Catalyzed by the Isochorismate-Pyruvate Lyase from Pseudomonas aeruginosa
Jose Olucha, Qianyi Luo and Audrey L. Lamb

P13. Directed Evolution of a Thermostable Quorum-Quenching Lactonase from the Amidohydrolase Superfamily

Jeng Yeong Chow, Bo Xue, Robert C. Robinson and Wen Shan Yew

P14. Studying the Substrate Tolerance of 4-Coumarate-CoA ligase from Streptomyces Coelicolor
Maybelle K. Go, Lim Yan Ping and Yew Wen Shan

P15. Synthesis and Screening a Haloacetamide Containing Library
Justin E. Jones, Jessica Slack, Corey P. Causey and Paul R. Thompson

P16. Activity-Based Protein Profiling of Protein Arginine Methyltransferase 1
Obiamaka Obianyo, Corey P. Causey and Paul R. Thompson

P17. Characterizing the Mechanism of PRMT1 Catalysis Using Site Directed Mutagenesis
Heather Rust and Paul Thompson

P18. The Development and Use of Clickable Activity Based Protein Profiling Agents for Protein Arginine Deiminase 4
Jessica L. Slack, Corey P. Causey, Yuan Luo and Paul R. Thompson

P19. Control of Mitochondrial Biogenesis by Mitochondrial Dynamics

Tianzheng Yu and Yisang Yoon

P20. Kinetic Analysis of Malate Synthase from
Mycobacterium tuberculosis
Christine Quartararo and John S. Blanchard

P21. Hydroxymethylglutaryl-CoA lyase (HMGCL): Functional insights from structures of HMGCL-Mg
++-hydroxyglutaryl-CoA and HMGCL R41M-Mg++-HMG-CoA ternary complexes
Henry M. Miziorko, Zhuji Fu, Jennifer A. Runquist and Jung-Ja P. Kim

P22. A Heme-dependent Tyrosine Hydroxylase
Katherine L. Connor and Barbara Gerratana

P23. The Structure of a C-3'-Methyltransferase Required for the Biosynthesis of dTetronitrose
Nathan A. Bruender, James B. Thoden, Manpreet Kaur, Marie K. Avey and
Hazel M. Holden

P24. Structural and Functional Studies of the Dehydrogenases Required for the Biosynthesis of 2,3-Diacetamido-2,3-dideoxy-d-mannuronic Acid
James B. Thoden and Hazel M. Holden

P25. Design of Non-covalent Small Molecule Inhibitors for Granzyme B
Mi-Sun Kim, Dean A Heathcote, Philip G Ashton-Rickardt and James P. Snyder

P26. Long-range dynamic networks in the function and fidelity of poliovirus RNA-dependent RNA polymerase

David D. Boehr, Xiaorong Yang and Jesse L. Welch

P27. Kinetically Controlled Drug Resistance: How
Penicillium brevicompactum Survives
Xin Sun, Bjarne Gram Hansen and Lizbeth Hedstrom

P28. Spectroscopic Studies on the [4Fe-4S] Cluster in Adenosine 5-phosphosulfate Reductase from Mycobacterium tuberculosis
Devayani P. Bhave, Jiyoung A. Hong, Michael Lee, Wei Jiang, Carsten Krebs and Kate S. Carroll

P29. Dihydrofolate reductase: A correlation between the donor-acceptor distance and its fluctuation to the catalyzed hydride transfer

Vanja Stojkovic, Laura Perissinoti, Stephen J. Benkovic and Amnon Kohen

P30. Characterization of the Carboxybiotin Intermediate in Reactions of Pyruvate Carboxylase.
Tonya N. Zeczycki, Martin St. Maurice, Sarawut Jitrapakdee, John C. Wallace, Paul V. Attwood and W. W. Cleland

P31. Mechanistic Studies of Methylphosphonate Synthase, a Non-Heme Fe- Dependent Dioxygenase Linked to the Marine Carbon-Phosphorus Cycle
Heather A. Cooke and Wilfred A. van der Donk

P32. Structural and Functional Significance of the Unstructured Loop Region of Alkanesulfonate Monooxygenase
Jingyuan Xiong and Holly R Ellis

P33. Kinetic and Chemical Mechanisms of Homocitrate Synthase from
Thermus thermophilus
Vidya Prasanna Kumar and Paul F. Cook

P34. Solvent Isotope Effects on the Desulfonation Mechanism of the Two-Component Alkanesulfonate Monooxygenase System
John M. Robbins and Holly R. Ellis

P35. Developing polyketide-based inhibitors targeting 3-dehydroquinate dehydratase in the shikimate biosynthesis pathway.
Vivian W. N. Cheung, Maybelle K. Go, Robert C. Robinson and Wen Shan Yew

P36. Structural studies of MppR: An enzyme of unknown function from enduracididine biosynthesis

Tyler Voegtline, Bilal H. Sayyed, Eric D. Lund and
Nicholas R. Silvaggi

P37. On the Analysis of Ligand Displacement Kinetics when a Probe is Rate Contributing
Hidehisa Iwata and Mark S. Hixon

P38. Discovery and design of inhibitors of dimethylarginine dimethylaminohydrolase inhibitors by high throughput screening
Thomas Linsky, Corey Johnson, Yun Wang and Walter Fast

P39. Substrate Specificity Amongst β-Ketoacyl-ACP Synthases
Janine Borgaro, Andrew Chang and Peter J. Tonge

P40. Coenzyme-induced conformational changes in a flavin-dependent N-hydroxylating monooxygenase
Pablo Sobrado, Wyatt Chocklett, Elvira Romero and Michelle Oppenheimer

P41. Histidine 252 in Adenosine 5'-phosphosulfate Reductase Is Required for Substrate Binding

Jiyoung A. Hong and Kate S. Carroll

P42. Characterization and purification of 3-deoxy-d-manno-oct-2-ulosonic acid hydroxylase, KdoO from Burkholderia ambifaria
Hak Suk Chung and Christian R. H. Raetz

P43. 4-Oxalocrotonate Decarboxylase and Vinylpyruvate Hydratase from Leptothrix cholodnii SP-6 May Act on a Chlorinated Form of the Substrate: Analysis and Quantitation
Stephanie Taylor and Christian P. Whitman

P44. Identification of the allosteric response element(s) in the active site of mammalian pyruvate kinase isozymes
James Urness, Cody Timmons, Kelly Thuet and
Aron W. Fenton

P45. Characterization of substrate binding loop of 3α-hydroxysteroid dehydrogenase/carbonyl reductase
Chi-Ching Hwang, Yu-Mei Su and Hsin-Wei Chen

P46. Promotion of Oligomeric DNA Polymerase Complex Formation Dictates Alternative Enzymatic Activities in Archaea
Michael A. Trakselis, Zhongfeng Zuo, Hsiang-Kai Lin and Robert Bauer

P47. MshB – A Cambialistic Metallohydrolase?

M. Hernick, X. Huang and E. Kocabas

P48. Probing the mechanism of DpgC, a unique cofactor and metal-free dioxygenase involved in the biosynthesis of vancomycin
Heather Condurso, Elisha Fielding, Paul Widboom and Steve Bruner

P49. Functional Promiscuity of
Rhodospirillum rubrum RuBisCO for RuBisCO-Like Protein Reactions
Benjamin Warlick and John A. Gerlt

P50. Thymidylate Synthase Catalyzed H-Transfers: Role of Tunneling and Dynamics in Different Enzyme catalyzed C-H activations

Zhen Wang and
Amnon Kohen

P51. Elucidating the Energetics of Active Site Loop Motion in Formyl-CoA:Oxalate CoA Transferase

Sangbae Lee, Mengen Chen, Wei Yang and
Nigel G. J. Richards

P52. Discovery of a New Class of Quiescent Affinity Label: 4-Halopyridines Inactivate Dimethylarginine Dimethylaminohydrolase

Corey M. Johnson, Thomas W. Linsky, Joyce Er and
Walter Fast

P53. Mechanism of a disulfide-generating enzyme: the Quiescin-sulfhydryl oxidase from
Trypanosoma brucei
Vamsi K. Kodali, Benjamin A. Israel and Colin Thorpe

P54. Two mechanisms for UDP-2,3-diacylglucosamine hydrolysis in lipid A biosynthesis

Hayley E. Young, Louis E. Metzger IV, John K. Lee, Robert M. Stroud and Christian R. H. Raetz

P55. Enzymatic Study and Chemical Inhibitor of the Histone Acetyltransferase Tip60
Jiang Wu, Nan Xie, Emilia N. Elangwe, Chao Yang and Yujun George Zheng

P56. Engineering
E. coli for new chemical function
Brooks B. Bond-Watts and
Michelle C. Y. Chang

P57. Characterization of enzymes involved in the degradation of 2-aminoethylphosphonate in
Sinorhizobium meliloti: phosphonoacetaldehyde dehydrogenase PhnY and phosphonoacetate hydrolase PhnA
Svetlana A. Borisova, Vinayak Agarwal, Nurul Zulkepli, Satish K. Nair, Wilfred A. van der Donk and William W. Metcalf

P58. Suppression of a polymerase general acid mutant: Consequences on replication speed and polymerase fidelity
Jamie J. Arnold, Madhumita Yennawar, Yan Zhao, Eric D. Smidansky and Craig E. Cameron

P59. Identification of PODANSg2158 from P. anserina as a Nitroalkane Oxidase and Comparison with the Enzyme from F. oxysporum
José R. Tormos and Paul F. Fitzpatrick

P60. Inhibition by Nitric Oxide of Catalysis by Oxalate Decarboxylase

Mario Edgar G. Moral, Chingkuang K. Tu, Witcha Imaram, Alexander Angerhofer, David N. Silverman and Nigel G. J. Richards

P61. Studies on the kinetic sequence of the Mycobacterium tuberculosis DHFR
Clarissa M. Czekster, An Vandemeulebroucke and John S. Blanchard

P62. Design, Synthesis and Evaluation of Quinolone-based Inhibitors of
E. coli PBP5
A. G. Shilabin, B. Jayaram and R. F. Pratt

P63. Inhibition of Bacterial Phosphopantothenoylcysteine Synthetase
James D. Patrone, Jiangwei Yao, Jennifer L. Meagher, Jeanne A. Stuckey, and
Garry D. Dotson

P64. Determination of the crystal structure of a two-domain NRPS fusion protein, EntEB, from the enterobactin synthetic cluster of Escherichia coli
Jesse A. Sundlov, Eric J. Drake, Courtney C. Aldrich and Andrew M. Gulick

P65. Probing the Mechanism of Phenylalanine Hydroxylase Using Rapid-Mixing Techniques with High-Resolution Mass Spectrometry
Kenneth M. Roberts and Paul F. Fitzpatrick

P66. Identification of the Enzyme That Catalyzes the Final Step in the Biosynthesis of Queuosine
Zachary D. Miles, Reid M. McCarty, Gabriella Molnar and Vahe Bandarian

P67. The chemistry of DXP synthase: Implications for selective inhibitor design

Francine Morris and
Caren L. Freel Meyers

P68. The Parts Aren’t Interchangeable: Installing a Proton-Transfer Network into a Class 1A Dihydroorotate Dehydrogenase

Claudia A. McDonald and Bruce A. Palfey

P69. Characterization of the Putative Diels-Alderase LovB CON

Joel J. Bruegger, Brian Ames, Suzanne Ma, Jesse Li, John Vederas, Yi Tang and Sheryl Tsai

P70. Structural Analysis of the Isochorismate-Pyruvate Lyase from Pseudomonas aeruginosa
Andrew Ouellette, Jose Olucha, Qianyi Luo, Roberto De Guzman and Audrey L. Lamb

P71. A Site Under Selective Pressure in the LexA Repressor is Required for Control of the DNA Damage Response
D. C. Marciano, P. Katsonis, A. K. Adikesavan, C. Herman and O. Lichtarge

P72. Characterization of
Ceriporiopsis subvermispora Bicupin Oxalate Oxidase Expressed In Pichia pastoris
Kelsey Uberto, Eric Hoffer , Patricia Moussatche, Alexander Angerhofer, Witcha Imaram, Nigel G. J. Richards and Ellen W. Moomaw

P73. Chemical Model for the Action of a Single Catalytic Residue, Arginine, at Active Site of Uroporphyrinogen Decarboxylase
Charles A. Lewis, Jr. and Richard Wolfenden

P74. Auxiliary Proteins in Alginate Maturation

Emma K. Farrell, Krista Arnett, Howard Robinson, Joel T. Weadge, P. Lynne Howell and Peter A. Tipton

P75. Assessment of Invertebrate Thiaminases by Mass Spectrometry
Ivy Bergquist, Randy L. Sears Jr., Lisa C. Harding, Vatsal M. Patel, Seetha R. Thummuru and John V. Schloss

P76. Mechanism-based Affinity Capture of Sirtuins
Jessica Falco, Yana Cen, Dou Yeon Youn, Ping Xu and Anthony A. Sauve

P77. Confirmation of the mechanism of Unsaturated Glucuronyl Hydrolase
S. A. K. Jongkees and S. G. Withers

P78. Design, synthesis and evaluation of new -lactamase inhibitors
Ronak Tilvawala and R. F. Pratt

P79. Boronate Inhibitors of Penicillin Binding Proteins: Evaluation
in vitro, in Cell Membranes, and in vivo
Liudmila Dzhekieva and R. F. Pratt

P80. Human mitochondrial RNA polymerase: Evaluation of the single-nucleotide-addition cycle on synthetic RNA/DNA scaffolds
Eric D. Smidansky, Jamie J. Arnold, Ibrahim M. Moustafa, Shelley L. Reynolds and Craig E. Cameron

P81. Dynamics of Conformational Heterogeneity within the Michaelis Complex of Lactate Dehydrogenase
Ruel Z. Desamero, Beining Nie, Nick Zhadin, Hua Deng and Robert Callender

P82. An Unprecedented Mechanism in Thiamine-Dependent Enzyme Catalysis: DXP Synthase

Leighanne A. Brammer, Jessica Mott, Herschel Wade and Caren Freel Meyers

P83. Ultra High Resolution Analysis of CTX-M
β-Lactamase to study catalysis and discover novel inhibitors
Derek Nichols and Yu Chen

P84. Characterization and Lead Development of inhibitors of Dimethylarginine Dimethylaminohydrolase

Gayle Burstein , Thomas W. Linsky and Walter Fast

P85. Transition State of Human Nicotinamide Phosphoribosyltransferase
Emmanuel S. Burgos, Mathew J. Vetticatt and Vern L. Schramm

P86.
15N Isotope Effects on NH3 for the reaction of NAD+ Synthetase (M. tuberculosis)
Laurie A. Reinhardt, Kaiti Chang, Barbara Gerratana and W. Wallace Cleland

P87. Computational design of enzymes for novel carbon-carbon bond formation reactions
Asha Chaubey, Elizabeth Noey and K. N. Houk

P88. Exploring The Inhibitory Effect Of The Hydrazine Functionality On The Histone Demethylase LSD1

Shonoi A. Barnett, Lindsay Avery, Rong Huang and Philip A. Cole

P89. Inhibition of Serine/Amine Amidohydrolases by O-Aryloxycarbonyl Hydroxamates and Oxathiazol-2-ones: Penicillin acylase and -lactamases
S. A. Adediran and R. F. Pratt

P90. Substrate specificity of penicillin-binding proteins
V. V. Nemmara and R. F. Pratt

P91. Coupled pKa Shift Between Conserved Cysteine and Aspartate Plays Key Role in Protein Splicing
Zhenming Du, Yangzhong Liu and Chunyu Wang

P92. Enzymatic Mechanism of Protein Arginine Methyltransferase (PRMT) Probed by Stopped-Flow Fluorescence
You Feng and Y. George Zheng

P93. Global Dynamics in Catalysis by Horse Liver Alcohol Dehydrogenase?
Bryce V. Plapp, Atsushi Yahashiri, Rachel S. Wallace, Karthik K. Shanmuganatham and Timothy J. Herdendorf

P94. DNA translocation dynamics revealed by single molecule nucleotide binding kinetics
Matthew Kellinger, Jonas Korlach and Kenneth A. Johnson

P95. Allosteric Regulation of α-Isopropylmalate Synthase from Mycobacterium tuberculosis
Ashley K. Casey, Jordyn L. Johnson and Patrick A. Frantom

P96. Enzymology as a Tool for Crystallography within the CSGID
Misty L. Kuhn, Magdalena M. Makowska-Grzyska, George Minasov, Ludmilla Shuvalova, Ievgeniia Dubrovska, James Winsor, Andrzej Joachimiak and Wayne Anderson

P97. High-Throughput Screening for Selective Inhibitors of N5-Carboxyaminoimidazole Ribonucleotide Mutase
Hanumantharao Paritala, Maria Fawaz, James B. Thoden, Hazel M. Holden and Steven M. Firestine

P98. Long-distance relationships: Communication between the active site lid and a remote loop in PEPCK
Sarah M. Holyoak and Todd Holyoak

P99. Site-specific installation of two lysine derivatives in histone H3
Wenshe Liu, Ying Huang and Wei Wan

P100. Targeting thymidylate biosynthesis for the development of new antibiotics
Eric M. Koehn, Scott A. Lesley, Irimpan I. Mathews and Amnon Kohen

P101. 15N, 13C Primary and 18O Nucleophilic Kinetic Isotope Effects for the Reaction Catalyzed by Sir2
Mark A. Anderson, W. W. Cleland, Brian C. Smith, Dawei Chen and John M. Denu

P102. Functional Assignment of the N245 Residue of 4-Hydroxyphenylpyruvate Dioxygenase from
Streptomyces avermitilis: A Key Residue in Hydroxylation Regiospecificity
June Brownlee, Dhara Shah, Judith Bates and
Graham R. Moran

P103. Small-molecule inhibitor of a human deubiquitylating enzyme complex

Junjun Chen, Thomas Dexheimer, Yongxing Ai, Anton Simeonov and
Zhihao Zhuang

P104. Insights into the chemical mechanism of eukaryotic UDP-galactopyranose mutases
Michelle Oppenheimer, Jin Qi, Janice Llanos-Valezquez and Pablo Sobrado

P105. Substrate Identification for the HDAC orthologue Rpd3 using
in vivo photo-crosslinking
Noah Wolfson, Jody Lancia, Anna Mapp and Carol Fierke

P106. Kinetically Controlled Allosteric Activation: Potassium creates a ball and socket joint in IMP Dehdyrogenase
Thomas V. Riera, Lianqing Zheng, Helen R. Josephine, Donghong Min, Wei Yang and Lizbeth Hedstrom

P107. The Human Hotdog-fold Protein THEM4 is Both a Protein Kinase B
α (Akt1) Regulator and a Fatty Acyl-CoA Thioesterase
Hong Zhao, Anthony Choudhry, Jeffery D. Carson, Michael Schaber, Peter J. Tummino, Lusong Luo, Marco Bossoffi and Debra Dunaway-Mariano

P108. Insights Into the Divergence of Function in the Hotdog-fold Protein Products of Gene Duplicates II:
A Case Study of the E. coli Hotdog Thioesterases YbdB (EntH) and YdiI
John Latham, Rui Wu, Danqi Chen, Karen Allen and Debra Dunaway-Mariano

P109. Biotin synthase: How iron-sulfur clusters are used to make carbon-sulfur bonds
Andrew M. Taylor, Christine E. Farrar, Michael R. Reyda, Corey J. Fugate and Joseph T. Jarrett

P110. Thermodynamic Profiles for Tight Binding Inhibitors to MTANs

Keisha Thomas and Vern L. Schramm

P111. Replica Path Determination of Bortezomib Deboronation
Joseph D. Larkin, Peng Tao, H. Lee Woodcock, Milan Hodoscek and Bernard R. Brooks

P112. Biosynthesis of 1-Deoxynojirimycin
Lorraine Clark and Nicole Horenstein

P113. Detailed Kinetic Analysis of Irreversible EGFR Inhibitors as a Method of Driving Drug Design
Phillip A. Schwartz and Brion W. Murray

P114. Investigation of Substrate Recognition in the Yeast Candida albicans Protein Prenyltransferases
Elaina A. Zverina and Carol A. Fierke

P115. Role of dynamics in tuning fidelity of RNA-dependent RNA polymerase elucidated by molecular dynamics simulation
Ibrahim M. Moustafa, Coray M. Colina and Craig E. Cameron

P116. The Human Estrogen Sulfotransferase - a Half-Site Reactive Enzyme
Meihao Sun and
Thomas S. Leyh

P117. Real-time Fluorescence Polarization Assay for Steady-state Kinetics and Screening for Inhibitors of Bacterial RNase P
Xin Liu and Carol Fierke

P118. A Critical Pleiotropic Interaction Governing the Specificity of 5-epi-aristolocholene Synthase and its Evolutionary Implications
Troy Wymore and Charles L. Brooks III

P119. Pre-steady state analysis of trans-3-chloroacrylic acid dehalogenase
Jamison P. Huddleston, Gottfried K. Schroeder, Kenneth A. Johnson and Christian P. Whitman

P120. The rate-limiting step for the second-order hydrolysis of many substrates by acetylcholinesterase (kcat/KM) is gated entry to the catalytic triad
Jeffrey T. Auletta, Veena Beri, Robert Chapman, Juanita F. Wood, Abdul H. Fauq and Terrone L. Rosenberry

P121. Enzyme Diversity and Evolution among the Enolase Superfamily: Understanding Sequence/Function/Structure Relationship
Ayano Sakai, Alexander A. Fedorov, Elena V. Fedorov, Steven C. Almo and John A. Gerlt

P122. QueE, a New Member of the Radical SAM Superfamily, is Involved in the Biosynthesis of 7-deazapurines
Reid M. McCarty and Vahe Bandarian

P123. Evidence for a phosphorylated enzyme intermediate in the reaction catalyzed by methylthioribose-1-phosphate isomerase
Vamsee Veeramachineni and Andrew S. Murkin

P124. Reactions of Propiolic Acid with Tautomerase Superfamily Dehalogenases: Analysis and Implications

Gottfried K. Schroeder, William H. Johnson, Jr., Jamison P. Huddleston, Kenneth A. Johnson, and Christian P. Whitman

P125. Structural evidence that peroxiredoxin catalytic power is based on transition state stabilization
Leslie B. Poole, Andrea Hall, Derek Parsonage and P. Andrew Karplus

P126. Selective Inhibition of Ras Function in Live Cells using Caged Farnesyltransferase Inhibitors
Daniel Abate-Pella, Nicholette A. Zeliadt, Timothy M. Dore, David A. Blank, Elizabeth V. Wattenberg and Mark D. Distefano

P127. Investigation of the HAD Superfamily Phosphatidic Acid Phosphatase Human Lipin1 and its HAD Superfamily Phosphatase Regulator Dullard
Jeremiah Farelli, Rui Wu, Megan Garland, Li Zheng, Debra Dunaway-Mariano and Karen Allen

P128. Insights Into the Divergence of Function in the Hotdog-fold Protein Products of Gene Duplicates II: A Case Study of the Human Hotdog Thioesterases hTHEM5 and hTHEM4
Hong Zhao, Dennis Dominguez, Kap Lam, Osnat Herzberg and Debra Dunaway-Mariano

P129. Identification and Structure Determination of Novel Adenine Deaminase Enzymes
Alissa M. Goble, Zhening Zhang, Subramanyam Swaminathan and Frank M. Raushel

P130. A Study on the Transesterification of Methyl Aryl Phosphorothioates in Methanol Promoted by Cd(II), Mn(II) and a Synthetic Pd(II) Complex
David R. Edwards, Alexei Neverov and R. Stan Brown

P131. Impact of temperature on the time required for primordial chemistry and enzyme evolution
Randy B. Stockbridge, Charles A. Lewis, Jr., Yuan Yang and Richard Wolfenden

P132. Is Leu403 an Absolute in Maintaining the “V” Conformation of Thiamin Diphosphate in Benzoylformate Decarboxylase?

Forest H. Andrews and Michael J. McLeish

P133. Structure and Mechanism of Benzaldehyde Dehydrogenase: A Class 3 Aldehyde Dehydrogenase from
Pseudomonas putida
Michael J. McLeish, Shreenath Prasad, Megan P.D. Zahniser, Gregory Petsko and Dagmar Ringe

P134. Enzymatic Extender Unit Generation for
In Vitro Polyketide Synthase Reactions: Structural and Functional Showcasing of Streptomyces coelicolor MatB
Amanda Jane Hughes and Adrian Keatinge-Clay

P135. Diketide Substrates Probe the Biocatalytic Potential of Modular Polyketide Synthase Ketoreductases
Shawn K. Piasecki, Clint A. Taylor, June Liu, Jianting Zheng, Joshua F. Detelich, Arkady Komsoukaniants, Dionicio R. Siegel and Adrian T. Keatinge-Clay

P136. Guilt by association: Functional annotation of TM0486 from
Thermotoga maritime by identification of its bound ligands
Mark J. Snider, Brad A. Palanski and Zachary Rotter

P137. Aerobic catabolism of nicotinic acid in
Bordetella bronchiseptica: Mechanistic studies of NicC, NicX and NicF
Mark J. Snider, Eric Sullivan, Matthew Henke, Virginia Kincaid, Ryan Story and Roger Rowlett

P138. Challenging a Paradigm. Catalysis and Inhibition in Cysteine Proteases
Michael Shokhen, Netaly Khazanov and
Amnon Albeck

P139. Necrotic cell death: Is it manageable?

Keren Schultz, Boris Khalfin, Ilana Nathan and
Amnon Albeck

P140. Computational Investigation of the Cisoid Cyclization Pathway of 5-epi-aristolocholene synthase

Carlos A. Ramirez-Mondragon and Troy Wymore

P141. Computational Study of Glutamate-
γ-Semialdehyde Oxidation Catalyzed by Thermus Thermophilus Gamma Glutamyl Semialdehyde Dehydrogenase
Nikolay Simakov and Troy Wymore

P142. Structural investigation of a novel NRPS cluster from
Pseudomonas aeruginosa
Carter A. Mitchell and Andrew M. Gulick

P143. Computational Exploration of Reaction Mechanism: Energetics Of DD-Peptidase Using A Hybrid QM/MM With The Off-Path Simulation
Justin K. White, Jacquline Hargis and H. Lee Woodcock

P144. The role of Phe161 in the mechanism of NADH-dependent persulfide reductase from
Shewanella loihica PV-4
Kyu Hyun Lee and E. J. Crane

P145. On the accuracy of NMR-measured protein p
Ka values
Helen Webb, Barbara Mary Tynan-Connolly, Gregory M. Lee, Damien Farrell, Fergal O'Meara, Chresten R. Søndergaard Kaare Teilum, Chandralal Hewage, Lawrence P. McIntosh and
Jens Erik Nielsen

P146. Crystal Structures of Both Unbound and ATP Analog-Bound Forms of
E. coli Anhydromuramic Acid Kinase, An Important Enzyme in Peptidoglycan Recycling
C. Leigh Allen, Bradley M. Hover, Nathan I. Nicely and Dewey G. McCafferty

P147. Identifying the Protein Targets of Epidermal Growth Factor-Induced Hydrogen Peroxide

Candice E. Paulsen and Kate S. Carroll

P148. Substrate Recognition and Catalytic Mechanism of MenB, the 1,4-Dihydroxynaphthoate Synthase in the Menaquinone Biosynthesis Pathway
Huei-Jiun Li, Xiaokai Li, Nina Liu, Huaning Zhang and Peter J. Tonge

P149. Insights into the Mechanism of a Bacterial Quercetin 2,3 Dioxygenase

Sara Bowen, Matthew R. Schaab and
Wilson A. Francisco

P150. Enzymatic Incorporation of Bioorthogonal Function into Proteins: New Strategies for Microarray Construction
Rajesh Viswanathan
and Alden E. Voelker

P151. Kinetic Studies of the Bi-Functional Enzyme a-Aminoadipate-Semialdehyde-Synthase from Bovine Liver
William E. Karsten
and Paul F. Cook




logo4c_sm
Organizing Committee
John Richard, Chair SUNY Buffalo
Kay Ahn Pfizer Global Research and Development
Tadhg Begley Texas A&M University
Debra Dunaway-Mariano University of New Mexico
Francine Perler New England Biolabs
Richard Wolfenden University of North Carolina at Chapel Hill

John P. Richard
Tina L. Amyes
University at Buffalo
Department of Chemistry
Buffalo, NY 14260, USA
(716) 645 4232 (John)
(716) 645 4231 (Tina)
Fax: (716) 645 6963